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On the next day, muscle tissue sections were incubated with anti-mouse secondary antibody IgG2b (A 21242), IgG1 (A 21120) and IgM (A 21426) and wheat germ agglutinin (WGA) Alexa Fluor 488 conjugate (Thermo Fisher Scientific, 0.004 mg/mL) with 2.5% goat serum in 1X PBS for 1 hour at RT followed by PBS wash

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It should subside within a week, and its important to stay hydrated by drinking plenty of water and limit alcohol intake during this time

Patients with atypical hyperinsulinism (defined as having no identified genetic mutation and with mosaic histomorphology) had significantly higher post-prandial plasma GLP-1 concentration when compared to other forms of hyperinsulinism (29)